https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49443 Wed 28 Feb 2024 14:45:02 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50275 Wed 12 Jul 2023 15:07:08 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39628 Pseudomonas fluorescens group and Brachypodium distachyon, a model for economically important food, feed, forage, and biomass crops of the grass family. We collected and analyzed root exudates of B. distachyon and demonstrated the presence of multiple carbohydrates, amino acids, organic acids, and phenolic compounds. The subsequent screening of bacteria by Biolog Phenotype MicroArrays revealed that many of these metabolites provide carbon and energy for the Pseudomonas strains. RNA-seq profiling of bacterial cultures amended with root exudates revealed changes in the expression of genes encoding numerous catabolic and anabolic enzymes, transporters, transcriptional regulators, stress response, and conserved hypothetical proteins. Almost half of the differentially expressed genes mapped to the variable part of the strains' pangenome, reflecting the importance of the variable gene content in the adaptation of P. fluorescens to the rhizosphere lifestyle. Our results collectively reveal the diversity of cellular pathways and physiological responses underlying the establishment of mutualistic interactions between these beneficial rhizobacteria and their plant hosts.]]> Wed 10 Aug 2022 11:58:55 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44763 Pseudomonas is a large and diverse genus of Gammaproteobacteria. To provide a framework for discovery of evolutionary and taxonomic relationships of these bacteria, we compared the genomes of type strains of 163 species and 3 additional subspecies of Pseudomonas, including 118 genomes sequenced herein. A maximum likelihood phylogeny of the 166 type strains based on protein sequences of 100 single-copy orthologous genes revealed thirteen groups of Pseudomonas, composed of two to sixty three species each. Pairwise average nucleotide identities and alignment fractions were calculated for the data set of the 166 type strains and 1224 genomes of Pseudomonas available in public databases. Results revealed that 394 of the 1224 genomes were distinct from any type strain, suggesting that the type strains represent only a fraction of the genomic diversity of the genus. The core genome of Pseudomonas was determined to contain 794 genes conferring primarily housekeeping functions. The results of this study provide a phylogenetic framework for future studies aiming to resolve the classification and phylogenetic relationships, identify new gene functions and phenotypes, and explore the ecological and metabolic potential of the Pseudomonas spp.]]> Tue 08 Nov 2022 11:16:28 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35003 Tue 03 Sep 2019 17:52:20 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53066 Fri 17 Nov 2023 11:52:33 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49376 Fri 12 May 2023 14:13:07 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42774 Fri 02 Sep 2022 11:00:23 AEST ]]>